SBF allows us to obtain high resolution 3D images of large volume from a sample on a scanning electron microscope (SEM). You can analyze the 3D organization of your tissue (volume, distance, polarity…).
At the BIC, we offer training and expertise on sample preparation with NCMIR clearing protocol. For image acquisition, we propose assistance and training on GeminiSEM300 from Zeiss with SBF Tri-View from Gatan.
3D reconstruction of mitochondria network in muscular cell, Pasdois & Bevilacqua (BIC)