Photonic Microscopy Unit
| Services | Techniques | Quantity | € HT Academic | € HT Private sector or non-nationals |
|---|---|---|---|---|
| Sample preparation (only for 2 trials) | Clearing (i-DISCO or CUBIC) | Per sample | 25 | 50 |
| Expansion Microscopy | Per sample | 10 | 20 | |
| Training (price per user) | Clearing (i-DISCO or CUBIC) and Expansion Microscopy | 4h | 120 | 240 |
| Microscopy | 3h | 120 | 240 | |
| Upgrade | 1.5h | 60 | 120 | |
| Analysis software* | 1h | 40 | 80 | |
| Use of systems | Widefield microscopy and Macroscopy | 1h | 12 | 24 |
| Confocal microscopy and TIRF | 1h | 24 | 48 | |
| Widefield or confocal microscopy + FRAP or LIFA | 1h | 24 | 48 | |
| Multiphoton or lattice light-sheet microscopy | 1h | 48 | 96 | |
| STED microscopy | 1h | 60 | 120 | |
| Image analysis (commercial softwares) | 1h | 6 | 12 | |
| Imaris satellite license | 1 day | 18 | 36 | |
| Slice preparation | 1h | 6 | 12 | |
| Engineer service | Image acquisition | 1h | 240 | 480 |
| Image analysis (contact us for a quote) | 1h | 30 | 60 |
*Charged analysis software trainings for Imaris, PALM Tracer, Tesseler and PoCA.
Degressive rates proposed on the facility are (applied for each 4-month billing period) :
50% discount on widefield and confocal microscopy from 100 hours of acquisition.
50% discount on 2-photon and LLS microscopy from 40 hours of acquisition.
30% discount on overnight (from 8 pm to 8 am) and over the weekends acquisitions (from Friday at 8 pm to Monday 8 am).
The specific rates of the slide scanner Nanozoomer are the followings :
| Acquisition mode | Type | € HT Academic | € HT Private sector or non-nationals |
|---|---|---|---|
| Training | 1.5 hours | 60 | 120 |
| Single | Per hour | 18 | 36 |
| Batch | From 0 to 10 slides | 60 | 120 |
| From 11 to 20 slides | 108 | 216 | |
| From 21 to 30 slides | 156 | 312 | |
| From 31 to 50 slides | 192 | 384 | |
| From 51 to 100 slides | 240 | 480 | |
| From 101 to 210 slides | 360 | 720 |
Electron Microscopy Unit
| Prestations | Techniques | Type | € HT Academic | € HT Private sector or non-nationals |
|---|---|---|---|---|
| Sample preparation | Conventional sample preparation | By sample | Contact us | Contact us |
| Ultra-thin sections with double positive contrast | By sample | Contact us | Contact us | |
| Negative staining | By sample | Contact us | Contact us | |
| High pressure freezing | 10 samples | Contact us | Contact us | |
| Cryosubstitution | 10 samples | Contact us | Contact us | |
| Cryo-sectionning | By sample | Contact us | Contact us | |
| Fixation-deshydratation for SEM | By sample | Contact us | Contact us | |
| Dessication | By sample | Contact us | Contact us | |
| Metal Sputtering | By 6 samples | Contact us | Contact us | |
| Immunostaining | Contact us | Contact us | ||
| Use of systems | Transmission electron microscopy 120kV (H7650) | 1h | 50 | 70 |
| Transmission electron microscopy 200kV (Talos) | 1h | 70 | 100 | |
| Scanning electron microscopy (Gemini SEM300 / Quanta 200) | 1h | 50 | 70 | |
| Cryo-SEM (Gemini SEM300) | 1h | 55 | 80 | |
| Serial Block Face Imaging (Gemini SEM300) | 1h | contact us | contact us | |
| Ultramicrotome | 1h | 10 | 15 | |
| Cryo-ultramicrotome (UC7-FC7) | 1h | 35 | 50 | |
| Training | Electron microscopy | Flat rate | 250 | |
| Upgrade | 1h | 50 | ||
| Theoric | 1h | 60 |
Plant Imaging Unit
| Type | Equipment or technique | Duration | € HT Academic | € HT Private sector or non-nationals |
|---|---|---|---|---|
| Sample preparation | Chemical fixation | 1 sample type | 20 | 40 |
| HPF ICE Leica | 5 shoots | 80 | 160 | |
| AFS | 5 days | 150 | 300 | |
| Immuno-labelling | 5 grids / 1 slide | 15 | 30 | |
| Vibrated blade Microtome | 1h | 8 | 16 | |
| Microtome Microm 35S | 1h | 11 | 22 | |
| Ultra-microtome UC7 (diamond from PIV) | 1h | 20 | 40 | |
| Ultra-microtome UC7 (diamond from user) | 1h | 12 | 24 | |
| INTAVIS | 1 batch | Contact us | Contact us | |
| Photonic microscopy | Macroscope Zeiss Axiozoom | 1h | 8 | 16 |
| Widefield microscope Zeiss AxioImager 2 | 1h | 14 | 28 | |
| Confocal microscope Zeiss LSM 880 | 1h | 27 | 54 | |
| Fluorocam | batch | Contact us | Contact us | |
| Electron microscopy | TEM FEI Tecnai Spirit 120kV – Conventional | 1h | 27 | 54 |
| TEM FEI Tecnai Spirit 120kV – Tomography | 5h | 126 | 252 | |
| TEM FEI Tecnai Spirit 120kV – Cryo | 5h | 140 | 280 |