30 Jun The BIC welcomes international users and publishes with them

As part of its involvement in national and international infrastructures and initiatives (France BioImaging, NeuBIAS and Euro BioImaging), the Bordeaux Imaging Centre regularly welcomes in its premisses external users. As a results of such activities, our engineers are involved in research projects which are validated by publications. Recently, two projects led to papers in eLife and Bone.

The first one involves a collaboration between the BIC and the lab of Wim Annaert (VIB-KU Leuven Center for Brain & Disease Research) that aims to study the diffusion properties of gamma secretase complex components at the cell surface. Abril Escamilla Ayala (PhD student) was first funded by FBI for a short stay to perform the proof of concept experiments in May 2019. She then obtained a funding from the FEBS to come back for 2 intense weeks of experiments in July 2019. Thanks to the brand new cell culture room of the BIC and to the PALM system, Abril was able to work on her own cell lines, to learn  how to acquire and analyse SPT data. Since then exchanges about data analysis, interpretation, and paper writing continued and the paper was accepted during the lockdown. The paper should be soon available on eLife website.

Super-resolution microscopy reveals majorly monodisperse presenilin1/γ 1 -secretase at the cell surface

Abril A Escamilla-Ayala, Ragna Sannerud, Magali Mondin, Karin Poersch, Wendy Vermeire, Laura Paparelli, Caroline Berlage,Marcelle Koenig, Lucía Chávez-Gutiérrez, Maximilian H Ulbrich, Sebastian Munck, Hideaki Mizuno, Wim Annaert

The second one results from a Short Term Scientific mission supported by NeuBIAS. It consisted in a 2 weeks stay for Marco Tarasco, a PhD student from the BioSkel Lab (Faro, Portugal), who learned ImageJ Macro programming. He has developed a toolset to characterize Zebrafish opercular bone, scales and caudal fin rays, as readouts for effects of water pollutants. His toolbox is freely available from GitHub, and the paper describing our work is out on Bone journal

ZFBONE: An ImageJ toolset for semi-automatic analysis of zebrafish bone structures

Marco Tarasco, Fabrice P. Cordelières,  M., Leonor Cancela, Vincent Laizé