30 Mar Maxime participated in his first conference by presenting a talk about “Active Image Optimization for Lattice Light-Sheet Microscopy” at FOM21
On March 29, 2021, Maxime participated in his first conference by presenting a talk about “Active Image Optimization for Lattice Light-Sheet Microscopy” at FOM21. The theme of the session was the development of techniques for light sheet microscopy.
He presented his work on the integration of adaptive optics in the detection path of the lattice light-sheet microscope of the platform. This project aims at correcting the detection beam of optical aberrations that can occur in the depth of the samples and degrade the image resolution. The process consists of an “Autofocus” followed by a “3N method”, two process based on image optimization. The “Autofocus” allows to move precisely the light-sheet at the detection objective focal plane to take high resolution images. The “3N method” permits to maximize a merit factor on the image by applying a coefficient to a deformable mirror for each aberrations function. The sensibility of “3N method” is based on image resolution, that’s why the “Autofocus” is made in first.
This process has been applied on live organotypic brain slice containing neurons labelled with cytosolic fluorescent proteins. The figure show that the active image optimization allows to keep a high resolution and intensity even in depth of the sample.
3D reconstruction (deconvolved) of neurons in live organotypic brain slice at 64 µm beside the sample surface, before and after optimization. On raw data, a plot intensity was made on dendrites and spines to highlight the resolution and intensity gain of active image optimization on LLSM.