Designation

Widefield Cryo

Model

Leica EM-Cryo-CLEM

Objectives

Leica HCX PL APO 50X / 0.90 CLEM

Software

Matrix MAPS module in LAS X

Filter Cubes

DAPI / GFP / TRITC

General informations

Cryo-chamber and cryo-transfer shuttle for cryogenic imaging. For single 3 mm electron microscopy grids and Autoloaderâ„¢ sample loader from FEI system.
Motorized stage in X and Y to acquire mosaics of images
Greyscale sCMOS camera Hamamatsu Flash4.0 LT+ for fluorescence

Acquisition Modes

Transmission and fluorescence with the greyscale camera in cryogenic conditions
Mosaics of images (scan-slide)

Applications

CLEM cryo-workflow
Cryo-fluorescence acquisition on vitrified samples

Category
Photonic imaging