Designation
Video-microscope
Manufacturer
Leica
Model
Leica DMI8
Specifications
Inverted Leica DMi8 stand, equipped with environmental control (37°C and CO2) and a hardware-based focus maintaining system (AFS).
Objectives
10x/0.32 dry (phase contrast), 20x/0.4 dry (phase contrast), 40x/0.6 dry (phase contrast), 63x/1.4 oil (DIC), 100x/1.4 oil (DIC). If you want to use an objective absent from this list, look at the complete list of objectives.
Excitation
CoolLED pE4000 illumination device (365nm, 38510nm, 405nm, 435nm, 460nm, 470nm, 490nm, 500nm, 525nm, 550nm, 580nm, 595nm, 635nm, 660nm, 740nm, 770nm).
Motorized filter wheels
simples: DAPI/FITC/TRITC; doubles: GFP/mCherry, CFP/YFP; Quadruples: DAPI/FITC/TRITC/CY5, CFP/YFP/RFP/CY7
Stage
Motorized stage in XYZ and galvanometric stage for fast and precise z-stack acquisitions
Camera
Large field, highly sensitive sCMOS camera Hamamatsur Flash 4.0 v2
Software
Microscope controled by Metamorph software
Applications
Fixed or live samples with a thickness up to 50 µm
Acquisition modes
Widefield in fluorescence and in transmission on fixed and live samples