Designation
Video-spinning-disk-FRAP microscope
Model
Leica DMI6000B
Objectives
10X phase, 20X dry, 40X oil, 63X oil, 100X oil (If you want to use an objectif absent from this list, look at the complete
list of objectives)
4 diodes laser
408 nm, 491 nm, 561 nm, 638 nm
Software
Microscope controled by Metamorph software
Light
Illumination by LEDs which allows 7 excitation range (for DAPI, CFP, GFP, YFP, TRITC, mCherry, CY5)
General informations
Motorized stage in XYZ and p iezo-stage for precise and fast z-stack acquisitions
Inverted microscope with environmental control at 37°C and CO2 provider.
System of FRAP
Widefield
1 resolutive camera Photometrics HQ2
Spinning-disk
Confocal unit Yokogawa CSU-X1 with 2 camera output
1 very sensitive EMCCD camera Photometrics Quantem 512 + 1 resolutive CCD camera Photometrics HQ2
Motorized filter wheels (simple, dual and quad bands filters allowing fast multi-colors acquisitions)
Acquisition modes
Widefield and confocal in fluorescence and in transmission on fixed and lived samplesPhase microscopy
Applications
Fixed or live samples with a thickness up to 100 µm