Designation
TIRF-PALM-STORM microscope
Model
Nikon Ti-Eclipse
Diodes laser
405 nm (100 mW), 488 nm (150 mW), 532 nm (1 W), 561 nm (200 mW), 642 nm (1 W).
Objectives
20x 0.5 dry, 40x 1.3 oil, 100x 1.49 oil (TIRF)
Detector
EMCCD Camera Photometrics Evolve (512x512 chip, pixel 16µm)
General informations
iLas2 TIRF arm + FRAP
Motorized stage XYZ with PFS autofocus system
Applications
TIRF microscopy (evanescent waves)
Super-resolution microscopy PALM or dSTORM or uPAINT under widefield or TIRF illumination