Microscope 2-photon in-vivo
1 LED source (with 4 LEDs), 4 filters sets (DAPI, GFP, mCherry and Cy5 filters), 1 sCMOS camera (Hamamatsu Orca Flash 2.8)
3 femtosecond lasers: Coherent Chameleon Vision 2, Coherent Chameleon Ultra 2 & NEW Spark Alcor 1064 nm usable simultaneously with 2 scanners (1 resonant & 1 galvanometric) for simultaneous imaging and photomanipulation.
2 positions objective slider (available objectives: Nikon 5X dry, Olympus 20X 1.0 W & Nikon 25X 1.1 W)
2 upper GaAsP NDD detectors + 2 lower GaAsP NDD detectors, with GFP and mCherry filter sets. 1 trans PMT with Dodt contrast
Motorized stage allowing to use tissue slices or entire animals (mouse or rat). Anesthesia system available for in vivo imaging.
Z-stack imaging: Objective piezo system for a fast and precise scan in z with the possibility to scan 3D regions of interest
Different devices triggered by the microscope: Electrophysiology devices (2 motorized micromanipulators Luigs and Neumann, Amplifier and Digitizer Axon Instruments, Master 8), 1 external LED at 595 nm with optical fibers for opto-genetics experiments, 1 picospritzer.
Acquisitions in 2P mode on galvo scanner with/without photomanipulation and electrophysiology
Acquisitions in 2P mode on resonant scanner with/without photomanipulation and electrophysiology
Acquisitions in widefield mode on sCMOS camera
Observation of tissue slices or entire animals
Photomanipulation: uncaging, photo-activation, optogenetic, etc...
Possible coupling with electrophysiology for electrical stimulation or Ephys recording