Designation

Microscope 2-photon in-vivo

Model

Femtonics FEMTO3D-RC

Microscope equipped with

1 LED source (with 4 LEDs), 4 filters sets (DAPI, GFP, mCherry and Cy5 filters), 1 sCMOS camera (Hamamatsu Orca Flash 2.8)

3 femtosecond lasers: Coherent Chameleon Vision 2, Coherent Chameleon Ultra 2 & NEW Spark Alcor 1064 nm usable simultaneously with 2 scanners (1 resonant & 1 galvanometric) for simultaneous imaging and photomanipulation.

2 positions objective slider (available objectives: Nikon 5X dry, Olympus 20X 1.0 W & Nikon 25X 1.1 W)

2 upper GaAsP NDD detectors + 2 lower GaAsP NDD detectors, with GFP and mCherry filter sets. 1 trans PMT with Dodt contrast

Motorized stage allowing to use tissue slices or entire animals (mouse or rat). Anesthesia system available for in vivo imaging.

Z-stack imaging: Objective piezo system for a fast and precise scan in z with the possibility to scan 3D regions of interest

Different devices triggered by the microscope: Electrophysiology devices (2 motorized micromanipulators Luigs and Neumann, Amplifier and Digitizer Axon Instruments, Master 8), 1 external LED at 595 nm with optical fibers for opto-genetics experiments, 1 picospritzer.

Acquisition modes

Acquisitions in 2P mode on galvo scanner with/without photomanipulation and electrophysiology

Acquisitions in 2P mode on resonant scanner with/without photomanipulation and electrophysiology

Acquisitions in widefield mode on sCMOS camera

Applications

Observation of tissue slices or entire animals

Photomanipulation: uncaging, photo-activation, optogenetic, etc...

Possible coupling with electrophysiology for electrical stimulation or Ephys recording