01 Jul A new color for the lattice microscope
We have recently added a new laser line in the laser combiner of the lattice light-sheet microscope. A 442 nm laser, 100mW, from Oxxius was mounted and aligned in the combiner. You can now use 405, 442, 488, 560 and 642 nm wavelengths for imaging or photostimulation.
Owing to this 442 nm line you can achieve efficient excitation of blue fluorophores such as CFP, Alexa Fluor 430 or ATTO 465. This channel can be combined with a green, red or far red channel for specific labelling of 4 different structures.
With 442 nm light you can also be photoactivate CaMK II, via the light driven LOV2 domain.