New features on the Femtonics 2-photon microscope
1983
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New features on the Femtonics 2-photon microscope

As you may know, the Bordeaux Imaging Center has 2 microscopes performing 2-photon (2P) microscopy. This technique allows, by using an infrared femtosecond laser, to obtain an emission of fluorescence in the depth of thick living samples.

Since 2017, our Femtonics system was able to do 2P imaging by using a galvo scanner (raster scan, linescan, etc…) or a resonant scanner (fast raster scan) with the femtosecond laser Chameleon Vision 2. Last year, following the installation of an anesthesia station by isoflurane, the first images in vivo have been obtained.

Now, since last week, a second femtosecond laser Chameleon Ultra 2 has been aligned in the optical pathway. And coupled to the latestversion of the Femtonics software MESc, this allows to perform:

  • 2 colors 2-photon imaging simultaneously, to look at green and red fluorochromes at the same time
  • 2P imaging coupled with 2P photo-manipulation, to activate biological processes by the light

This second modality can be done thanks to the simultaneous use of the 2 scanners: imaging is done on the very fast resonant scanner and photo-manipulation on the very precise galvo scanner to activate molecules, by uncaging or optogenetics, in a drawn-by-user region of interest.

Of course, these experiments can still be done on tissue slices but also on the mouse, and triggered with electrophysiological measurements or any other device.

Don’t hesitate to contact Sebastien () if you are interested to try this very powerful system for your own experiments.

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