Videomicroscope
527
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Designation

Video-microscope

Manufacturer

Leica

Model

Leica DMI8

Specifications

Inverted Leica DMi8 stand, equipped with environmental control (37°C and CO2) and a hardware-based focus maintaining system (AFS).

Objectives

10x/0.32 dry (phase contrast), 20x/0.4 dry (phase contrast), 40x/0.6 dry (phase contrast), 63x/1.4 oil (DIC), 100x/1.4 oil (DIC). If you want to use an objective absent from this list, look at the complete list of objectives.

Excitation

CoolLED pE4000 illumination device (365nm, 38510nm, 405nm, 435nm, 460nm, 470nm, 490nm, 500nm, 525nm, 550nm, 580nm, 595nm, 635nm, 660nm, 740nm, 770nm).

Motorized filter wheels

simples: DAPI/FITC/TRITC; doubles: GFP/mCherry, CFP/YFP; Quadruples: DAPI/FITC/TRITC/CY5, CFP/YFP/RFP/CY7

Stage

Motorized stage in XYZ and galvanometric stage for fast and precise z-stack acquisitions

Camera

Large field, highly sensitive sCMOS camera Hamamatsur Flash 4.0 v2

Software

Microscope controled by Metamorph software

Applications

Fixed or live samples with a thickness up to 50 µm

Acquisition modes

Widefield in fluorescence and in transmission on fixed and live samples

Category
Photonic imaging