Spinning-disk LIFA microscope
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Designation

Spinning-disk LIFA microscope

Model

Leica DMI6000B

Objectives

10X dry, 20X dry, 40X oil, 63X oil, 100X oil (If you want to use an objective absent from this list, look at the complete list of objectives)

Software

Microscope controled by Metamorph software

Confocal unit

Yokogawa CSU-X1 with 2 camera output

4 diodes laser

408 nm, 491 nm, 561 nm, 638 nm

General informations

Motorized stage in XYZ and galvanometric stage for fast and precise z-stack acquisitions
Inverted microscope with environmental control at 37°C and CO2 or carbogen provider
Systeme of FRAP
Autofocus control system (Hardware autofocus - AFS)
Spinning-disk
1 very sensitive EMCCD camera Photometrics Quantem 512 + 1 resolutive CCD camera Photometrics HQ2
Motorized filter wheels (simple, double and quad bands filters allowing fast multi-color acquisitions)
LIFA (FLIM imaging)
1 module of modulated LEDs + 1 modulated laser (trough the spinning-disk)
1 intesified camera LiCAM2

Acquisition modes

Widefield and confocal in fluorescence and in transmission on fixed and live samples
Measure of fluorescence lifetime on fixed or live samples (in widefield or confocal)

Applications

Fixed or live samples with a thickness up to 100 µm

Category
Photonic imaging