Confocal microscope SP8-STED
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Designation

Confocal microscope SP8-STED

Model

Leica DMI6000 TCS SP8 X

Specifications

Diode laser 405 nm + white laser 470-670nm
STED lasers : 592 (cw), 660 (cw), 775 (pulsed)
Motorized stage in X, Y and Z  + galvanometric stage for fast and precise z-stack acquisitions
STED module : 2D and/or 3D

Objectives

10X dry, 20X dry, 20X multi-immersion (= IMM), 40X oil, 63X oil, 100X oil (If you want to use an objective absent from this list, look at the complete list of objectives)

Scanner tandem

1 standard scanner (10 Hz to 1800 Hz) + 1 resonnant scanner (8000 Hz)

Detectors

2 PMTs, 2 hybrid detectors, 1 PMT Trans

Applications

- Acquisitions on cultured cells or thick tissues in fluorescence (up to 100 µm thickness)
- Acquisitions on material (in reflection mode)
- STED on cultured cells or thin tissue slices

Acquisition modes

- Confocal acquisitions in transmission (brightfield or DIC), fluorescence (colocalisation, 3D reconstruction)
- Spectral acquisition (at excitation and emission)
- Acquisition in reflection
- Mosaics of images acquisitions in 2D or 3D

Category
Photonic imaging