Designation
Confocal microscope SP5-MP
Model
Leica DM6000 TCS SP5 MP
Objectives
10X 0.4 dry, 20X 0.7 multi-immersion, 40X 1.25 oil, 63X 1.4 oil, 25X 0.95 water (eventually 40X 0.8 water or 63X 0.9 water if needed)
Laser
405 nm, Argon (458, 476, 488, 496, 514 nm), 561 nm, 594 nm, 633 nm
Scanner tandem
1 standard scanner (10 Hz to 1800 Hz) + 1 resonant scanner (8000 Hz)
Detectors
5 internal PMT whose 2 hybrid detectors, 2 Hybrid NDD, 1 PMT trans
General informations
IR pulsed Coherent Chameleon Ultra 2 tunable between 680 and 1080 nm Motorized stage X, Y and Z + galvanometric Z-stage for precise and fast z-stack acquisitions
Acquisition modes
Confocal acquisitions in transmission (brightfield or DIC), fluorescence (colocalisation, 3D reconstruction) 2-photon mode Spectral acquisition (at excitation and emission) Acquisition in reflection Acquisition of mosaics of images 2D or 3D
Applications
Acquisitions on cultured cells or thick tissues, fixed or alive, in fluorescence, in confocal mode (up to 100 µm of thickness) or in 2-photon mode (> 100 µm)Acquisitions on material (in reflection mode)