Lattice Light-sheet microscope
1198
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Designation

Lattice Light Sheet miroscope

Light Source

Laser combiner : 405 nm (300 mW), 488 nm (1 W), 560 nm (2 W), 642 nm (2 W)

Objectives

Excitation : Special Optics 0.65 NA, 3mm WD, water dipping Detection : Nikon 25X, 1.1 NA, 2mm WD, water dipping Inverted widefield fluorescence : Olympus 40X, 0.8 NA

Light sheet dimensions

0.5 µm thick by 15 µm long and 100 µm large (can be modified)

XYZ Resolution

270 nm x 270 nm x 550 nm (@ 488 nm)

Camera

Hamamatsu Orca Flash4 v2 sCMOS, pixel size 6.5 µm

Sample mounting

Sample mounted on 5 mm diameter glass coverslips

Photomanipulation module

Photomanipulation (FRAP, uncaging, photoactivation,...) at 405, 488, 560 or 642 nm targeted to user-defined ROIs via a pair of galvo-mirrors

Applications

Cell imaging, tissue imaging (max size ~ 1 mm3), live imaging, time-lapse, 3D dynamics

Category
Photonic imaging