AFS 1
Leica
Automated freeze substitution system
Temperature monitored between -150°C and +65°C
Liquid nitrogen is used for maintaining low temperature
An UV lamp allows resin polymerization
This system is used for low temperature dehydration of fixed biological samples, either by progressive lowering of temperature (PLT) or by freeze-substitution of frozen samples. AFS also allows resin polymerization at low temperature.